Does disk order matter?

I have an OWC Mercury Elite Pro Quad which is connecteed via USB-C to a Mac Studio (M1). I had a 4 x 4TB disk raid5 volume which I was using. I needed to use the enclosure for a project so I removed the 4 discs and installed new discs for my project. Now I want to pull the new discs and re-insert the 4 disk volume. In this process I never payed attention to the order of the disks.

Question 1: Does it matter if the drives don't go in the same slots as they were when i was originally using the raid volume? Can I insert them and continue to use them (and still access the data that's on that volume?)

Question 2: For the 'temp project' discs, I now want to re-purpose these to a Windows based PC. I tried inserting them into the PC but the PC is unable to initialize the discs. It sees an unrecognizable volume which cannot be removed and cannot create a volume. Did Softraid 'lock' those disks? Do I need to re-insert them into the OWC MEPQ and do something which will release them for other use? 

Thanks in advance

Phil